欧洲科学家利用WIWAM XY植物表型成像系统发表文章

欧洲科学家利用WIWAM XY植物表型成像系统发表文章 

来自欧洲的科学家，利用SMO公司构建的WIWAM XY植物表型成像系统，发表了题为Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling的文章。WIWAM XY作为一款通用的表型成像系统，已经广泛应用于各种小植物表型研究并发表了系列文章。文章就该系统有以下介绍：
 

多光谱植物表型
实验中利用该系统进行了拟南芥中的花青素和叶绿素含量测量(指数AriIdx和ChlIdx)并测量了与PSII最大量子效率相关的叶绿素荧光参数。所有数据都由可控环境中的自动植物表型成像平台-WIWAM XY 系统 (SMO, Eeklo, Belgium) 生成，该系统配备了6-Mp 16-bit 3CCD 顶视相机，相机安装在直角坐标机器人上，用于高通量和高分辨率荧光和多光谱成像。 

北京博普特科技有限公司是比利时SMO公司系列植物表型成像系统中国区总代理，全面负责其系列产品在中国市场的推广、销售和售后服务。

 


Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling

Abstract and Figures 
Rhizospheric microorganisms can alter plant physiology and morphology in many different ways including through the emission of volatile organic compounds (VOCs). Here we demonstrate that VOCs from beneficial root endophytic Serendipita spp. are able to improve the performance of in vitro grown Arabidopsis seedlings, with an up to 9.3-fold increase in plant biomass. Additional changes in VOC-exposed plants comprised petiole elongation, epidermal cell and leaf area expansion, extension of the lateral root system, enhanced maximum quantum efficiency of photosystem II (Fv/Fm), and accumulation of high levels of anthocyanin. Notwithstanding that the magnitude of the effects was highly dependent on the test system and cultivation medium, the volatile blends of each of the examined strains, including the references S. indica and S. williamsii, exhibited comparable plant growth-promoting activities. By combining different approaches, we provide strong evidence that not only fungal respiratory CO2 accumulating in the headspace, but also other volatile compounds contribute to the observed plant responses. Volatile profiling identified methyl benzoate as the most abundant fungal VOC, released especially by Serendipita cultures that elicit plant growth promotion. However, under our experimental conditions, application of methyl benzoate as a sole volatile did not affect plant performance, suggesting that other compounds are involved or that the mixture of VOCs, rather than single molecules, accounts for the strong plant responses. Using Arabidopsis mutant and reporter lines in some of the major plant hormone signal transduction pathways further revealed the involvement of auxin and cytokinin signaling in Serendipita VOC-induced plant growth modulation. Although we are still far from translating the current knowledge into the implementation of Serendipita VOCs as biofertilizers and phytostimulants, volatile production is a novel mechanism by which sebacinoid fungi can trigger and control biological processes in plants, which might offer opportunities to address agricultural and environmental problems in the future.