采用美国Sage Science公司 全自动核酸电泳和片段回收系统文章列表3
下列发表的文章采用美国Sage Science公司的Pippin Prep和Blue Pippin 全自动核酸电泳和片段回收系统或者从采用其技术或材料
环亚生物科技有限公司（APG BIO Ltd）是美国Sage Science公司在中国大陆的总代理商，详情请登录www.apgbio.com
April 2013
Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
Authors:
Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson
Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson
Info:
The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.
The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.
Citation:PLoS Comput Biol 9(4):e1003031
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April 2013
Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
Authors:
Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen
Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen
Info:
The Pippin Prep was used to size-selec Illumina RNA-seq libraries.
The Pippin Prep was used to size-selec Illumina RNA-seq libraries.
Citation:
Int. J. Mol. Sci. 2013, 14, 9080-9098
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Int. J. Mol. Sci. 2013, 14, 9080-9098
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April 2013
Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers
Authors:
Jakob Love, Heather A. Hoke, Charles Y. Lin, Ashley Lau, David A. Orlando, Christopher R. Vakoc, James E. Bradner, Tong Ihn Lee, and Richard A. Young
Jakob Love, Heather A. Hoke, Charles Y. Lin, Ashley Lau, David A. Orlando, Christopher R. Vakoc, James E. Bradner, Tong Ihn Lee, and Richard A. Young
Info:
The Pippin Prep was used to size-selec ChIP-seq libraries.
The Pippin Prep was used to size-selec ChIP-seq libraries.
Citation:Cell 153:320-334 April 2013
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March/April 2013
H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis
Authors:
Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh
Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh
Info:The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were found that shed new light on alternative mechanisms of methanogenic growth. The mutants were mapped by whole genome sequencing, in which Pippin Prep was used to size selec the libraries.
Citation:mBio (4)2:00062-13 March/April 2013
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January/February 2013
Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
Authors:
Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana
Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana
Info:
Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome sequence of one such strain. Nextera chemistry was used with size selection on the Pippin Prep to generate the sequencing libraries.
Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome sequence of one such strain. Nextera chemistry was used with size selection on the Pippin Prep to generate the sequencing libraries.
Citation:genomeA (1)1:e00144-12 January/February 2013
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January 2013
A genome-wide survey of genetic variation in gorillas using reduced representation sequencing
Authors:
Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith
Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith
Info:
The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion followed by size selection on the Pippin Prep for AluI fragments between 150-250 bp.
The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion followed by size selection on the Pippin Prep for AluI fragments between 150-250 bp.
Citation:pre-print
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January 2013
Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
Authors:
Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins
Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins
Info:
The authors designed a general method to study the arrangement of foreign DNA and host DNA rearrangements in trangenic animals. The protocol involves standard Illumina PE library generation, using the Pippin Prep for size selection, followed by microarray hybridization capture to isolate fragments containing the transgene.
The authors designed a general method to study the arrangement of foreign DNA and host DNA rearrangements in trangenic animals. The protocol involves standard Illumina PE library generation, using the Pippin Prep for size selection, followed by microarray hybridization capture to isolate fragments containing the transgene.
Citation:
Nucleic Acids Research, 2013, 1-5
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Nucleic Acids Research, 2013, 1-5
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January 2013
Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice
Authors:
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra
Info:
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prepwas used for size selection in their RADseq protocol.
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prepwas used for size selection in their RADseq protocol.
Citation:Letter, Nature 493:402-405
