4D-NucleofectorTM技术在肝细胞上的应用
以下文章来源于Lonza Bioscience ，作者Lonza Bioscience
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Laurent, V., Glaise, D., Nubel, T., Gilot, D., Corlu, A..Loyer, P.(2013).Highly Efficient SiRNA and Gene
Transferinto Hepatocvte-Like HepaRG Cells and
Primarv Human Hepatocytes: New Means for Drug
Metabolism and Toxicity Studies. In: Phillips, l.Shephard,E..Ortiz de Montellano.P.(eds)
Cytochrome P450 Protocols.Methods in Molecular Biology, vol987.Humana Press,Totowa,NJ.
https://doiorg/10.1007/978-1-62703-321-3 25
Tanner Rathbone.Ilavda Ates.Lawrence Fernando than Addlestone. Ciaran M.Lee,Vincent P.
Richards.and Renee N. Cottle.Electroporation-
Mediated Delivery of Cas9 RibonucleoproteinsResults in High Levels of Gene Editing in PrimaryHepatocytes.The CRISPR Journal.Jun 2022.397-409.
http;//doi.org/10.1089/crispr2021.0134
Chen,N.. Sivalingam,J.. Tan,S.et al. Plasmidelectroporated primary hepatocytes acquire quasi.physiological secretion of human insulin and
restore euglycemia in diabetic mice.Gene Ther 12,655-667(2005).
https://doi.org/10.1038/sigt.3302446
Tham CYL. Kah J.Tan AT Volz T Chia A. Giersch K.Ladiges Y, Loglio A, Borghi M, Sureau C,LamperticoP, Lutgehetmann M, Dandri M, Bertoletti A.
Hepatitis Delta Virus Acts as an ImmunogenicAdjuvant in Hepatitis B Virus-Infected Hepatocytes
Cell Rep Med.2020 Jul21:1(4):100060.
https://doi.org/10.1016/ixcrm.2020.100060
AlV AS.Mikolaiczak SA.Rivera HS.Camargo NJacobs-Lorena V, Labaied M, Coppens l, Kappe SHTargeted deletion of SAPl abolishes the expressionof infectivity factors necessary for successful
malaria parasite liver infection.Mol Microbiol.2008 Jul:69(1):152-63.
https://doi.org/10.1111/i1365-2958.2008.06271.X.Epub 2008 May 5.
