采用全自动核酸电泳和片段回收系统发表文章列表1

下列发表的文章采用美国Sage Science公司的Pippin Prep和Blue Pippin 全自动核酸电泳和片段回收系统或者从采用其技术或材料：

October 2013

Semiconductor-based DNA sequencing of histone modification states

Authors:
Christine S. Cheng, Kunal Rai, Manuel Garber, Andrew Hollinger, Dana Robbins, Scott Anderson, Alyssa Macbeth, Austin Tzou, Mauricio O. Carneiro, Raktima Raychowdhury, Carsten Russ, Nir Hacohen, Jeffrey E. Gershenwald, Niall Lennon, Chad Nusbaum, Lynda Chin, Aviv Regev & Ido Amit

Info:
Researchers at the Broad Institute and collaborators at several institutions provide optimized sample preparation protocols for the generation of ChIP-seq libraries on the Ion Torrent PGM. They showed that Pippin size selection was required to generate usable libraries, even down to sub-nanogram input. Results were comparable to results from Illumina Chip-Seq workflow.

Citation:
Nature Communications 4:2672

http://dx.doi.org/10.1038/ncomms3672
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October 2013

Mitochondrial Sequence Variation in African-American Primary Open-Angle Glaucoma Patients   (Pippin Prep)

Authors:
David W. Collins, Harini V. Gudiseva, Benjamin T. Trachtman, Matthew Jerrehian, Thomasine Gorry, William T. Merritt III, Allison L. Rhodes, Prithvi S. Sankar, Meredith Regina, Eydie Miller-Ellis, Joan M.
O’Brien

Info:
Researchers at the University of Pennsylvania deep-sequenced mitochondrial DNA of patients with primary open-angle glaucoma, plus a control group for comparison. They testing a theory implicating variation in mtDNA in this disease, but it could not be confirmed. Pippin Prep was used for size selection of Ion PGM libraries, per the Ion Express protocol.

Citation:PLoS ONE 8(10): e76627

http://dx.doi.org/10.1371/journal.pone.0076627
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October 2013

Genetic Basis for the Biosynthesis of the Pharmaceutically important Class of Epoxyketone Proteasome Inhibitors

Authors:
Michelle Schorn, Judith Zettler, Joseph P. Noel, Pieter C. Dorrestein, Bradley S. Moore, and Leonard Kaysser

Info:
Scientists from California and Germany teamed up to study genes that encode for the biosynthesis of an important component of certain proteasome inhibitors used for cancer treatment. The Ion PGM was used to sequence gene clusters in two microbial strains, reporting the first successful characterization of a region producing natural peptidyl-epoxyketones. For this project, libraries were prepared from genomic DNA sheared to 100 bp to 250 bp, after which sequencing material was separated and extracted using Pippin Prep.

Citation:ACS Chem. Biol. October 2013

http://dx.doi.org/10.1021/cb400699p
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October 2013

Improved Protocols for Illumina Sequencing

Authors:
Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow

Info:
A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow size distribution and Pippin automated size selection is discussed.

Citation:
Current Protocols in Human Genetics 18.2.1-18.2.42, October 2013

http://dx.doi.org/10.1002/0471142905.hg1802s79
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September 2013

Next-Generation Sequence Analysis of Cancer Xenograft Models

Authors:
Fernando J. Rossello, Richard W. Tothill, Kara Britt, Kieren D. Marini, Jeanette Falzon, David M. Thomas, Craig D. Peacock, Luigi Marchionni, Jason Li, Samara Bennett, Erwin Tantoso, Tracey Brown, Philip Chan, Luciano G. Martelotto, D. Neil Watkins

Info:
The authors looked at using xenografts as preclinical models for NGS cancer studies. The authors were able to show that mouse specific sequence was easily distinguished from human small cell lung cancer sequences. Pippin Prep size selection was used for whole genome library construction for the Illumina HiSeq.

Citation:PLoS ONE 8(9): e74432

http://dx.doi.org/10.1371/journal.pone.0074432
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August 2013

Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway

Authors:
Tao-Yi Cai, Wei Zhu, Xiong-Sheng Chen, Sheng-Yuan Zhou, Lian-Shun Jia and Yan-Qing Sun

Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China

Info:
From the Second Military Medical University in Shanghai, China, this qPCR gene expression study looks at the effect of fibroblast growth factor mesenchymal stems cells. The BluePippin was used to size-selec cDNAs.

Citation:Molecular Medicine Reports 8: 1323-1328, 2013

http://dx.doi.org/10.3892/mmr.2013.1668