信号通路筛选？LabEx抗体芯片实验服务

信号通路筛选？”芯“有灵犀一点通

 

 

找到信号通路的关键节点是复杂且耗时耗力的，抗体芯片试剂盒可以在一个实验室里监测信号通路中众多关键信号组分的变化，为您节省宝贵的时间和试剂！

 

 

每个试剂盒有2张玻片，每张玻片上有8或16张垫片，最多可同时检测32个样本，这样可使研究者在一次实验里有充分的自由度来检测多种浓度，细胞系或时间节点的样本！

 

抗体芯片基于夹心法ELISA原理进行设计，将众多信号通路关键节点蛋白的捕获抗体布于样品孔内做成抗体点阵，随后加入相应的识别抗体混合物，再利用两种不同的显色方式呈现结果，即化学发光法和荧光法检测！

 

目前的抗体芯片产品列表如下：

 

Products	荧光法		化学发光法
PathScan? Akt Signaling Antibody Array Kit	#9700	#9474
PathScan? EGFR Signaling Antibody Array Kit	#12785	#12622
PathScan? Intracellular Signaling Array Kit	#7744	#7323
PathScan? RTK Signaling Antibody Array Kit	#7949	#7982
PathScan? Stress and Apoptosis Signaling Antibody Array Kit（NEW）	#12923	#12856
PathScan? Th1/Th2/Th17 Cytokine Antibody Array Kit	#13124	#13047
PathScan? Immune Cell Signaling Antibody Array Kit（NEW）	#13788	#13792
PathScan? Cancer Phenotype Antibody Array Kit	#14822	#14821

 

自己不方便？我们来帮您！LabEx接受抗体芯片实验服务啦！

 

【实验室化学发光法检测仪器】

 

【实验室做出的结果照片及灰度值分析】

 

 注：全国服务都可接受，我们设有样本全国干冰运送冷链！

 

PathScan Akt Signaling Antibody Array Kit
#9700（荧光法）& #9474（化学发光法）
32次检测，16个蛋白靶点，512个数据点

 

 

Akt Signaling

 

	Target	Phosphorylation Site
1	Positive Control	N/A
2	Negative Control	N/A
3	Akt	Thr308
4	Akt	Ser473
5	S6 Ribosomal Protein	Ser235/236
6	AMPKa	Thr172
7	PRAS40	Thr246
8	mTOR	Ser2481
9	GSK-3a	Ser21
10	GSK-3b	Ser9
11	p70 S6 Kinase	Thr389
12	p70 S6 Kinase	Thr421/Ser424
13	Bad	Ser112
14	RSK1	Thr421/Ser424
15	PTEN	Ser380
16	PDK 1	Ser241
17	Erk1/2	Thr202/Tyr204
18	4E-BP1	Thr37/46

 

PathScan EGFR Signaling Antibody Array Kit
#12785（荧光法）& #12622（化学发光法）
16次检测，24个蛋白靶点，384个数据点

 

EGFR Signaling

	Target	Phosphorylation Site
1	Positive Control	N/A
2	Negative Control	N/A
3	EGFR	Total
4	EGFR	Thr669
5	EGFR	Tyr845
6	EGFR	Tyr998
7	EGFR	Tyr1068
8	EGFR - L858R Mutant	Total
9	EGFR - E746-A750 Deletion	Total?
10	MEK1	Total
11	MEK2	Total
12	MEK1/2	Ser221
13	MEK1/2	Ser217/221
14	Stat3	Tyr705
15	HER2/ErbB2	Total
16	HER2/ErbB2	Tyr1196
17	HER2/ErbB2	Tyr1221/1222
18	Met	Total
19	Met	Tyr1349
20	Met	Tyr1234/1235
21	PLCY1	Total
22	PLCY1	Ser1248
23	Akt	Thr308
24	Akt	Ser473
25	Erk1/2	Thr202/Tyr204
26	PARP	Asp214 (Cleavage)

 

PathScan Intracellular Signaling Antibody Array Kit
#7744（荧光法）& #7323（化学发光法）
32次检测，18个蛋白靶点，576个数据点

 

Intracellular Signaling

 

	Target	Phosphorylation Site	Modification
1	Positive Control	N/A	N/A
2	Negative Control	N/A	N/A
3	ERK1/2	Thr202/Tyr204	Phosphorylation
4	Stat1	Tyr701	Phosphorylation
5	Stat3	Tyr705	Phosphorylation
6	Akt	Thr308	Phosphorylation
7	Akt	Ser473	Phosphorylation
8	AMPKa	Thr172	Phosphorylation
9	S6 Ribosomal Protein	Ser235/236	Phosphorylation
10	mTOR	Ser2448	Phosphorylation
11	HSP27	Ser78	Phosphorylation
12	Bad	Ser112	Phosphorylation
13	p70 S6 Kinase	Thr389	Phosphorylation
14	PRAS40	Thr246	Phosphorylation
15	p53	Ser15	Phosphorylation
16	p38	Thr180/Tyr182	Phosphorylation
17	SAPK/JNK	Thr183/Tyr185	Phosphorylation
18	PARP	Asp214	Cleavage
19	Caspase-3	Asp175	Cleavage
20	GSK-3b	Ser9	Phosphorylation

 

PathScan RTK Signaling Antibody Array Kit
#7949（荧光法）& #7982（化学发光法）
16次检测，39个蛋白靶点，624个数据点

 

Receptor Tyrosine Kinases

 

	Target	Phosphorylation Site	Family
1	EGFR/ErbB1	pan-Tyr	EGFR
2	HER2/ErbB2	pan-Tyr	EGFR
3	HER3/ErbB3	pan-Tyr	EGFR
4	FGFR1	pan-Tyr	FGFR
5	FGFR3	pan-Tyr	FGFR
6	FGFR4	pan-Tyr	FGFR
7	InsR	pan-Tyr	Insulin R
8	IGF-IR	pan-Tyr	Insulin R
9	TrkA/NTRK1	pan-Tyr	NGFR
10	TrkA/NTRK2	pan-Tyr	NGFR
11	Met/HGFR	pan-Tyr	HGFR
12	Ron/MST1R	pan-Tyr	HGFR
13	Ret	pan-Tyr	Ret
14	ALK	pan-Tyr	LTK
15	PDGFR	pan-Tyr	PDGFR
16	c-Kit/SCFR	pan-Tyr	PDGFR
17	FLT3/Flk2	pan-Tyr	PDGFR
18	M-CSFR/CSF-1R	pan-Tyr	PDGFR
19	EphA1	pan-Tyr	EphR
20	EphA2	pan-Tyr	EphR
21	EphA3	pan-Tyr	EphR
22	EphB1	pan-Tyr	EphR
23	EphB3	pan-Tyr	EphR
24	EphB4	pan-Tyr	EphR
25	Tyro3/Dtk	pan-Tyr	Axl
26	Axl	pan-Tyr	Axl
27	Tie2/TEK	pan-Tyr	Tie
28	VEGFR2/KDR	pan-Tyr	VEGFR

 

Signaling Nodes

 

	Target	Phosphorylation Site	Family
29	Akt/PKB/Rac	Thr308	Akt
30	Akt/PKB/Rac	Ser473	Akt
31	p44/42 MAPK (ERK1/2)	p44/42 MAPK (ERK1/2)	MAPK
32	S6 Ribosomal Protein	Ser235/236	RSK
33	c-Abl	pan-Tyr	Abl
34	IRS-1	pan-Tyr	IRS
35	Zap-70	pan-Tyr	Zap-70
36	Src	pan-Tyr	Src
37	Lck	pan-Tyr	Src
38	Stat1	pan-Tyr	Stat
39	Stat3	pan-Tyr	Stat

 

PathScan? Stress and Apoptosis Signaling Antibody Array Kit 
#12923（荧光法）#12856（化学发光法）
32次检测，19个蛋白八点，608个数据点

 

Stress Apoptosis Signaling

	Target	Site	Modification
1	Positive Control	N/A	N/A
2	Negative Control	N/A	N/A
3	P44/42 MAPK(ERK1/2)	Thr202/Tyr204	Phosphorylation
4	Akt	Ser473	Phosphorylation
5	Bad	Ser136	Phosphorylation
6	HSP27	Ser82	Phosphorylation
7	Smad2	Ser465/467	Phosphorylation
8	p53	Ser15	Phosphorylation
9	p38 MAPK

详情点击→ https://www.u-labex.com/detail-lx-pcrarray.html?mk_channel=SWQC

上海乐备实生物技术有限公司，简称乐备实(LabEx)，是上海优宁维生物科技股份有限公司旗下全资子公司，主要专注于提供多组学/多因子相关检测分析的实验服务专家，目前已扩展到单细胞测序、空间多组学、流式检测、电化学发光、液相微阵列、蛋白芯片、抗体芯片、PCR Array、ELISA、Elispot、多色免疫组化等30+个技术平台。已经为超过3000个客户提供服务，累计检测样本超过100万，受到了广大客户的赞赏。