病毒包装

The Virus Platform provided packaging services for a variety of viral types. The packaged viruses are classified two categories: DNA integrative viruses and DNA non-integrative viruses. DNA integrative viruses refer to retrovirus and classical lentivirus whereas DNA non-integrative viruses refer to modified lentivirus, baculovirus, adenovirus, adeno-associated virus and baculovirus. The viral titer and purity are guaranteed for effective infection for both cultured cells and tissues.

1. Classical Lentivirus and retrovirus

Lentivirus is a genus of slow viruses of the Retroviridae family, characterized by a long incubation period. Lentiviruses and retroviruses can deliver a significant amount of genetic information into the DNA of the host cell and have the unique ability among retroviruses of being able to replicate in non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. Like many other types of retroviruses, lentivirus and retrovirus are excellent research tools for stable introduction of a gene product into in vitro systems or animal models for stable expression. Another common application is to use a lentivirus or retrovirus to introduce  an ShRNA cassette (e.g., ShRNA) for stable gene silencing. The titer of lentiviruses and retroviruses ranges from 1x10⁶ to 1x10⁸ TU/ml.

2. Modified Lentivirus

To develop efficient transient transfection methods, Biowit has also established a modified lentiviral vector system by inactivating the integrative elements of classical lentivruses. The modified lentivirus can efficiently infect a variety of mammalian cells like classical lentivirus whereas its viral DNA does not integrate into the host genome, thus it serve as an excellent alternative for routine transient transfection while it can also avoid the integrative side effects of classical lentivirus. The titer of non-integrative lentiviruses ranges from 1x10⁶ to 1x10⁸ TU/ml.

3. Adenovirus

Adenoviruses are medium-sized (90–100 nm), nonenveloped icosahedral viruses composed of a nucleocapsid and a double-stranded linear DNA genome. It can infect various species of vertebrates. Adenoviruses possess a linear dsDNA genome and are able to replicate in the nucleus of mammalian cells using the host’s replication machinery. Adenovirus is used as a vehicle to administer targeted therapy, in the form of recombinant DNA or protein. The titer of packaged adenoviruses ranges from 1x10¹⁰-1x 10¹² pfu/ml.

4. Adeno-associated virus

Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. Moreover, virus infection causes a very mild immune response. AAV can infect both dividing and non-dividing cells These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise. Different AAV serotypes have demonstrated patterns for the target genes in terms of expression time and host selectivity.  The Biowit virus platform provides a variety of AAV2 serotypes including AAV2/1, AAV2/2, AAV2/3, AAV2/4, AAV2/5, AAV2/6, AAV2/7, AAV2/8 and AAV2/9. The titer of packaged AAV  ranges from 1x10¹¹-1x 10¹² vg/ml.

Biowit Technologies Ltd. is a high-tech company specialized in providing R&D services and related products in the biological field. As a technology-driven and customer-focused company, BioWit provides a broad and integrated service and product portfolio including molecular biology, virus packaging (e.g., adenovirus, AAV, lentivirus, retrovirus and baculovirus), protein expression, cell biology, stem cells and transgenic models. One of the milestones achieved by Biowit is its recent sucess in the development of a series of serum-containing media as well as serum-free  or animal -free media for stem cell culture, which are tailored for clinical applications. The core technology team members have engaged in research and development for many years in world-famous academic institutions or prestigious drug companies. Biowit has established collaboration with international prestigious research institutions. The worldwide collaboration enables Biowit to timely renew its biotechnologies (http://www.biowit.com)

    百恩维生物科技有限公司（BioWit Technologies），是一家专业从事生物技术研发并提供相关技术服务和产品的高新技术企业。公司目前提供的技术服务和产品主要包括分子生物学技术服务、腺病毒（Adenovirus)、慢病毒(Lentivirus)、腺相关病毒（AAV）、杆状病毒（Baculovirus)等包装技术服务、蛋白质表达与纯化技术服务（包括原核细菌、杆状病毒-昆虫细胞、哺乳动物细胞等）、高效转染试剂（HET Kit)、细胞因子(如hLif、mLif、aFGF、bFGF、EGF、TGFα、TGFβ等）、干细胞培养基(如胎胚干细胞培养基、间充质干细胞培养基等）、干细胞的分离和存储以及个体特异性诱导型多能干细胞的制备和存储, 其中干细胞无血清或无动物源培养是专门为临床打造和设计的快速扩增细胞的培养基同时又能很好地维持其干细胞的潜能。公司的长期目标是推出人类重大疑难疾病的干细胞治疗及基因治疗临床方案和预防措施，重点研究神经疾病、视网膜疾病、白血病、血友病、艾滋病和肝炎的干细胞治疗和基因治疗方案，及提供高效的预防动物或人的传染病、流行病的疫苗(http://www.biowit.com)。