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Design of the CT26-hEpCAM-LZ cell line
The CT26-hEpCAM-LZ clonal cell line expresses high levels of human EpCAM, and forms solid tumors in vivo. An optimized version of a luciferase-ZsGreen (LZ) fusion protein is also stably expressed for in vivo imaging and ex vivo tracking.
CT26-hEpCAM-LZ featuresExpression analysis by flow cytometry. Human EpCAM expression assessed in CT26.WT parental cell line (left), human control cell line (center), and CT26-hEpCAM cell line (right).
Wild-type Balb/c mice were injected (s.c.) with 1x106 CT26-LZ (left) or CT26-hEpCAM-LZ (right) cells. Tumor growth was measured every 2 to 3 days. Tumor size (mm3) = (width2 x length)/2.
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