产品详情:

产品名称:
Recombinant Human IL4R protein ,C- His Tag
英文全称:Recombinant Human IL4R protein ,C- His Tag
纯度(Purity):>90% as determined by SDS-PAGE
内毒素(Endotoxin level):Please contact with the lab for this information.
蛋白构建(Construction):A DNA sequence encoding the Human IL4R (Gln26-Glu342) was fused with the C-terminal His Tag
表达宿主(Host):Mammalian cells
种属(Species):Homo sapiens (Human)
预测分子量(Predicted Molecular Mass):36.93 kDa
制剂(Formulation):Supplied as solution form in PBS or lyophilized from PBS .
运输方式(Shipping):In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage):Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution):Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
电泳图(SDS-PAGE image)
背景(Background)
分子别名(Alternative Names):IL4R,CD124.
Note:For research use only .
核心作用:

产品名称:Recombinant Human IL4R protein ,C- His Tag
英文全称:Recombinant Human IL4R protein ,C- His Tag
纯度(Purity):>90% as determined by SDS-PAGE
内毒素(Endotoxin level):Please contact with the lab for this information.
蛋白构建(Construction):A DNA sequence encoding the Human IL4R (Gln26-Glu342) was fused with the C-terminal His Tag
表达宿主(Host):Mammalian cells
种属(Species):Homo sapiens (Human)
预测分子量(Predicted Molecular Mass):36.93 kDa
制剂(Formulation):Supplied as solution form in PBS or lyophilized from PBS .
运输方式(Shipping):In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage):Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution):Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
电泳图(SDS-PAGE image)
背景(Background)
分子别名(Alternative Names):IL4R,CD124.
Note:For research use only .
操作步骤:

一、开盖预处理
蛋白冻干粉 4℃平衡 30 min,12000 rpm 低温离心 5 min,将粉末离心至管底。
二、蛋白复溶
加入无菌纯水配制成 0.1~0.5 mg/mL 蛋白液(浓度不低于 0.1 mg/mL);室温静置 5~10 min,禁止剧烈涡旋、反复吹打,避免蛋白变性。
三、C-His 标签蛋白纯化
预冷 PBS 平衡 Ni-NTA 柱;样品以 0.5 mL/min 流速上样;30 mM 咪唑缓冲液洗除杂蛋白,250 mM 咪唑洗脱目标蛋白。
四、分装保存
洗脱蛋白经 0.22 μm 滤膜除菌,无菌管分装;-20~-80℃冻存,添加 0.1% BSA 或 5% 海藻糖稳定剂,避免反复冻融。
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