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| 销售商： 上海谷研实业有限公司 | 查看该公司所有产品 >> |
产品概述衔接蛋白β抗体是针对衔接蛋白家族β成员的特异性抗体，衔接蛋白通过介导蛋白质-蛋白质相互作用，参与细胞信号转导、内吞作用、细胞骨架重塑等多种生理过程，其功能异常与肿瘤发生、免疫疾病等相关。该抗体多以兔多克隆或单克隆形式存在，经过亲和纯化处理，具有高特异性和灵敏度，可与人类、小鼠、大鼠等物种的衔接蛋白β发生交叉反应。适用于免疫印迹（WB）、酶联免疫吸附实验（ELISA）、免疫组化（IHC-P/IHC-F）、免疫沉淀（IP）、免疫荧光（IF）等实验，能精准检测衔接蛋白β的表达水平及与其他蛋白的相互作用，为研究细胞信号转导机制及相关疾病发病机制提供重要工具。
产品基本信息中文名称：衔接蛋白β抗体
英文名称：Adaptor Protein β Antibody
别 名:beta Adaptin; Adaptin beta 1 (beta prime); β-Adaptin; Adaptin β1; Adaptin-β1; Adaptin beta 2 (beta); Adaptin beta 2; Adaptin, beta 2 (beta); Adaptor protein complex AP 1 beta 1 subunit; Adaptor related protein complex 1 beta 1 subunit; Adaptor related protein complex 1, beta 1 subunit; Adaptor related protein complex 2 beta 1 subunit; Adaptor related protein complex 2, beta 1 subunit; ADTB 1; ADTB 2; ADTB1; ADTB2; AP 1 complex subunit beta 1; AP 105A; AP 105B; AP 2 complex subunit beta 1; AP1 Beta; AP1 complex subunit beta 1; AP105A; AP105B; AP1B1; AP2 beta; AP2 complex subunit beta 1; AP2B1; AP2beta; BAM 22; BAM22; Beta adaptin; Beta prime adaptin; Beta1 adaptin; Beta2 adaptin; CLAPB 1; CLAPB 2; CLAPB1; CLAPB2; Clathrin assembly protein complex 1; Clathrin assembly protein complex 1 beta large chain; Clathrin assembly protein complex 2 beta large chain; Clathrin associated/assembly/adaptor protein large beta 1; Clathrin associated/assembly/adaptor protein large beta 2; DKFZp781K0743; Golgi adaptor HA1/AP1 adaptin beta subunit; Plasma membrane adaptor HA2/AP2 adaptin beta subunit; Plasma membrane adaptor HA2/AP2 adaptor beta subunit; Plasma membrane adaptor HA2/AP2.
研究领域:细胞生物 染色质和核信号 信号转导 细胞周期蛋白 转录调节因子 细胞分化
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应；(predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, )
产品应用:ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量:103kDa
细胞定位:细胞浆 细胞膜
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived from human beta Adaptin: 351-450/937
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Function:
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
抗体的制备过程
抗体的制备过程1. 免疫原的制备
普通的大分子蛋白，通过分子克隆构建载体并在大肠杆菌中进行诱导表达获得重组蛋白，纯化鉴定后可直接作为免疫原。
小分子蛋白或化合物等分子量小，需要偶联载体对该分子进行改造才能使其成为具有免疫原性的抗原，常见偶联载体如BSA、OVA、HAS等。
2. 免疫动物
常用于制备抗血清的动物有豚鼠、家兔、鸡、大小鼠等，大量生产时需要用到狗、绵羊、山羊等。
3. 免疫血清的收集
一般家兔、绵羊、山羊可采用静动脉采血，家兔、豚鼠、大鼠、鸡可采用心脏采血，家兔、山羊、绵羊可采用静脉采血。
4. 免疫血清的纯化与鉴定
得到的抗血清需要进一步的纯化，利用偶联了抗原的亲和柱进行层析，具有高效，特异性强，纯度高的特定。接着要鉴定纯化蛋白的含量、相对分子的质量、纯度以及特异性。
5. 免疫血清的保存
建议将抗体分装后进行保存。抗体一般比较稳定，在-80℃ ～-20 ℃可以保存约5年而不会影响效价，而真空干燥保存时间可以更久。保存前需经除菌并添加防腐剂。
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抗体制备通用注意事项无菌操作：全程严格无菌，避免细菌或真菌污染，尤其是细胞培养环节；
实验记录：详细记录免疫时间、抗原浓度、血清效价、融合率等数据，便于后续重复实验；
应急方案：提前储备备份的免疫动物和杂交瘤细胞，避免意外情况导致实验失败；
伦理合规：动物免疫需符合实验室动物伦理规范，避免不必要的动物痛苦。
手机版：衔接蛋白β抗体
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