当前位置 > 首页 > 技术文章> cells
按分类查找文章
-
654 次 细胞与基因治疗核心原料AllCells产品解析及常见问题解答 2026-4-1
AllCells：DLS旗下CGT细胞治疗原料标杆品牌摘要AllCells隶属于Discovery Life Sciences（DLS），是CGT领域核心原料标杆品牌，专注提供GMP/RUO级人源细胞原料，覆盖CAR-T等疗法研发至产业化全场景
-
2912 次 微环境多靶标蛋白分析系统升级前后功能对比 2022-10-21
深入分析组织微环境对于理解疾病生物学及其进展至关重要。过去几年，芯片流式技术ChipCytometry产品在组织免疫分析中展现出的巨大应用价值，已经得到了世界各地用户的广泛认可和肯定，这种先进的
-
17138 次 CellSens standard软件主要功能操作说明 2018-5-22
一、明场实时图像调节及拍照保存1.打开cellSens软件后，首先在摄像控制工具窗口中切换相机芯片为“彩色模式”，此时相机自动切换为彩色芯片进行明场图像采图；接下来点击“实时观
-
2986 次 脾脏、胸腺和淋巴结中单个核细胞分离和培养 2017-3-16
基本方案从小鼠脾脏、胸腺和淋巴结制备单个核细胞分离和培养。实验材料1.RPMI-5或DMEM-5完全培养基。2.新鲜分离的小鼠器官：≤6周龄的小鼠胸腺；6周至6个月龄的小鼠脾脏和淋巴结。3.60mm&time
-
2654 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr
-
3544 次 Culture of human venous smooth muscle cells 2012-5-23
Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f
-
3421 次 Isolation of human prostatic smooth muscle cells 2012-5-8
Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of
-
3115 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
-
1966 次 Isolation of papillary cells 2012-3-26
Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept
-
5872 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc
-
3532 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli
-
4110 次 Isolation of human primary ovarian surface epithelial cells 2011-12-12
Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut
-
1915 次 Isolation of human prostatic epithelial cells 2011-11-25
Isolation of human prostatic epithelial cells1.A small piece of tissue from each specimen was removed and minced. 2.The tissue was digested with collagenase overnight. 3.To remove
-
3658 次 Isolation of stromal vascular cells from human adipose tissue 2011-9-30
Isolation of stromal vascular cells from human adipose tissue Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal ste
-
3703 次 Isolation and cultivation of endothelial progenitor cells (EPCs) 2011-9-30
Isolation and cultivation of endothelial progenitor cells (EPCs) Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue rege
-
2545 次 Isolation of rodent pancreatic β cells 2011-8-1
Isolation of rodent pancreatic β cells 1.Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.Rat islets were isolated from mal
-
2712 次 Culture of human renal proximal tubule cells and renal cortical fibroblasts 2011-7-11
Culture of human renal proximal tubule cells and renal cortical fibroblasts 1.The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibrobla
-
2376 次 Isolation of human colonic epithelial cells (EC) 2011-6-21
Isolation of human colonic epithelial cells (EC)1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were
-
2640 次 Isolation and Culture of Human Liver Stem Cells 2011-4-1
Isolation and Culture of Human Liver Stem CellsMaterials and Methods1.Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy li
-
2407 次 FITC标记抗体-Marsshall氏法 2010-10-13
FITC标记抗体-Marsshall氏法材料：抗体球蛋白溶液、0.5mol/L pH9.0碳酸盐缓冲液、无菌生理盐水、异硫氰酸荧光素、1%硫柳汞水溶液、三角烧瓶（25—50ml）、冰及冰槽（或1000ml烧杯）、电磁搅拌器