18676 次用BRET方法研究活细胞中GPCR蛋白相互作用的基本注意事项 2013-1-15
K. M. Kroeger1, K. A. Eidne1, Bernd Hutter2简介:BRET已经成功的应用于研究哺乳动物细胞中GPCR同源和异源二聚体以及涉及到受体脱敏作用和交易的受体/β-arrestin相互作用(综述,1)。BRET的
3116 次Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
5872 次Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc