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3201 次 羊布病(Brucellosis)ELISA试剂盒操作步骤 2016-9-20
我司ELISA试剂盒品质保证，质量优，价格实惠，是您生物实验的首选，如有需要可与我司销售人员联系。本试剂仅供研究使用目的：本试剂盒用于测定羊血清，血浆及相关液体样本中羊布病(Brucellosis)
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5254 次 肿瘤外泌体RNA通过激活肺泡上皮TLR3招募嗜中性粒细胞促进肺预转移微环境形成 2016-8-11
肿瘤外泌体RNA通过激活肺泡上皮TLR3招募嗜中性粒细胞促进肺预转移微环境形成研究背景1889年，Stephen Paget通过对大量肿瘤的解剖学研究发现，特定的肿瘤细胞总是倾向于转移到特定的组织器官。假
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10754 次 如何有效分离提取cfDNA用于检测？ 2016-5-26
说起肿瘤、癌症，已经不再像多年前一样让谈癌色变，但仍旧为人们所恐惧。成功治疗肿瘤还是以早发现早治疗为主。那么尽早检测和发现肿瘤便是首当其冲的核心。液体活检(Liquid biopsy)的检测方法，
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7020 次 一年4篇Cell一作论文 大牛分享了10条科研经验 2016-2-25
罗纳德·韦尔博士（Ronald D. Vale）是加州大学旧金山分校（UCSF）的细胞与分子药理学教授。研究方向是驱动蛋白和动力蛋白分子马达。他创造了一个科研界的世界记录：一年内作为第一作者发 4 篇高
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8033 次 Wheaton细胞培养系列CELLine生物反应器工作原理及特点 2015-5-27
Wheaton的CELLine生物反应器采用细胞室与培养基室半透膜分离培养技术，突破了传统细胞培养的空气，营养物质，代谢抑制因子对细胞生长的制约。最大程度的模拟了细胞在有机体的生长环境。实现了高
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6556 次 人全血Foxp3实验步骤 2014-12-3
需要试剂 HumanRegulatoryTCellWholeBloodStainingKit（#88-8996-40） 包含组分： (1)、1XRBCLysisBuffer:200mL (2)、FlowCytometryStainingBuffer:600mL (3)、Fixation/PermeabilizationCon
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3378 次 Cell-Based ELISA简介以及应用 2014-9-11
1、Cell-Based ELISA 的优点： Cell-Based ELISA（基于细胞的 ELISA）是一种全新的ELISA技术，有两个最突出的优点：1.1不需要抽提蛋白、包被微孔板：细胞直接在微孔板里培养，待检测的时候，将细
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10478 次 血细胞形态学检查的重要意义 2014-6-28
血液一般检验取材容易、检测便捷，仍然是筛检疾病、遴选其他实验检查的首要程序。血细胞计数及血液形态学检查是血液检验项目中最基础、最常用的检验。血细胞形态学检验是检验医学的基础和核心，
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5936 次 T Cell Receptor 信号通路图 2012-7-23
The T Cell Receptor plays a key role in the immune system. The specificity of the receptor is governed by the binding site formed from the mature alpha and beta chains (shown here)
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4745 次 B Cell Receptor 信号通路图 2012-7-13
B cells produce immunoglobulins (Ig, antibodies) that specifically bind antigen molecules. B cells first produce a membrane-bound form of immunoglobulin, the B cell receptor, as pa
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2654 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr
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1827 次 Fluorescent Staining of Cells 2012-6-20
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2. PBS, solution A. Procedure:1. Fix a
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3544 次 Culture of human venous smooth muscle cells 2012-5-23
Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f
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3422 次 Isolation of human prostatic smooth muscle cells 2012-5-8
Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of
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3116 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
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1968 次 Isolation of papillary cells 2012-3-26
Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept
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2232 次 Isolation of lymphatic endothelial cells 2012-3-12
Dermal Cell Suspensions1.Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2.Dermal sheets were prepared by incubatio
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5872 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc
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3532 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli
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2553 次 Placental trophoblast and chorionic cell cultures 2011-12-16
Placental trophoblast and chorionic cell cultures 1.Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2.Term human placentae a