文章中,作者检测了包括内参蛋白GAPDH在内的13种白质使用Wes系统建立绝对定量检测模型
定量分析63例临床样本中13种白质的表达水平
文中作者阐述了使用Wes nano-immunoassay method进行此项临床研究的原因:
1.The recent development of a method based on the combination of capillary nano-electrophoresis with immunoassay (CNIA), also known as Simple Western, requires only very small amounts of sample to be able to measure protein expression 3,7. This technical advance makes it possible to analyze the expression of 50-100 proteins in a single MM sample.
使用微量样本,因而使得一份多发性骨髓瘤样本可以进行50-100个蛋白检测成为可能。
2.Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation
准确定量CD138分选细胞蛋白质表达
3.This makes the CNIA platform a fast, effective and accurate tool for exploring the impact of different proteins on MM survival and for investigating new protein biomarkers that could help predict the response to new drugs that directly target specific proteins.
建立快速,搞笑,准确的生物标志物发现的方法,用于预测新药的生物学反应