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PsaA 抗体
PsaA 抗体
英文名称:Anti-PsaA subunit of Photosystem I antibody总访问:2884
国产/进口:进口半年访问:178
产地/品牌:原装进口产品类别:抗体
规       格:PHY0053 最后更新:2019-10-16
货       号:PHY0053
CAS   号:
参考报价:3180/150ug
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem I (PSI) is a multisubunit complex that catalyses the final stage of the light reactions in chloroplasts. This protein complex has two main components forming its core complex, PsaA and PsaB. These two subunits bind most of the cofactors required for charge separation and electron transfer in PSI.

  Synonyms:

PsaA, Photosystem I P700 chlorophyll a apoprotein A1

  Immunogen:

KLH-conjugated synthetic peptide of PsaA derived from Arabidopsis thaliana ATCG00350.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

83 / 70 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Populus trichocarpa, Gossypium raimondii, Solanum tuberosum, Spinacia oleracea, Zea mays subsp. Mays, Sorghum bicolor, Setaria viridis, Triticum aestivum, Panicum virgatum, Hordeum vulgare subsp. Vulgare, Vitis vinifera, Cucumis sativus, Glycine max, Chlamydomonas reinhardtii, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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